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medulloblastoma cell lines hdfa  (ATCC)


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    ATCC medulloblastoma cell lines hdfa
    A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human <t>medulloblastoma</t> cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).
    Medulloblastoma Cell Lines Hdfa, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medulloblastoma cell lines hdfa/product/ATCC
    Average 99 stars, based on 2046 article reviews
    medulloblastoma cell lines hdfa - by Bioz Stars, 2026-03
    99/100 stars

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    1) Product Images from "Identification of a small molecule targeting EPLIN as a novel strategy for the treatment of pediatric neuroblastoma and medulloblastoma"

    Article Title: Identification of a small molecule targeting EPLIN as a novel strategy for the treatment of pediatric neuroblastoma and medulloblastoma

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-025-07876-7

    A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).
    Figure Legend Snippet: A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).

    Techniques Used: Drug discovery, Over Expression, Derivative Assay, Control, Colony Assay, Cell Culture



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    medulloblastoma cell lines hdfa  (ATCC)
    99
    ATCC medulloblastoma cell lines hdfa
    A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human <t>medulloblastoma</t> cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).
    Medulloblastoma Cell Lines Hdfa, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medulloblastoma cell lines hdfa/product/ATCC
    Average 99 stars, based on 1 article reviews
    medulloblastoma cell lines hdfa - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

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    A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).

    Journal: Cell Death & Disease

    Article Title: Identification of a small molecule targeting EPLIN as a novel strategy for the treatment of pediatric neuroblastoma and medulloblastoma

    doi: 10.1038/s41419-025-07876-7

    Figure Lengend Snippet: A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).

    Article Snippet: hTERT-immortalized retinal pigment epithelial cell line (Cat. #CRL-4000) and neuroblastoma cell lines SK-N-AS (Cat. #CRL-2137), SH-SY5Y (Cat. #CRL-2266), SK-N-SH, (Cat. #HTB-11), CHP-212 (Cat. #CRL-2137), IMR-32 (Cat. #CRL-127), SK-N-BE2 (Cat. #CRL-2271) and medulloblastoma cell lines HDFa (Cat. #PCS-201-012), D283 (Cat. #HTB-185) and Daoy (Cat. #HTB-186) was obtained from (ATCC, Manassas, VA, USA). hTERT, SK-N-AS, SH-SY5Y, SK-N-SH, CHP-212, IMR-32, SK-N-BE [ ] was maintained in 1:1 ratio of EMEM (ATCC, Cat. #30-2003) and Ham’s F-12 Nutrient Mix (Thermo Fisher Scientific, Waltham, MA, USA, Cat. #11765054) supplemented with 1% penicillin streptomycin (Thermo Fisher Scientific, Cat. #15140-122) and 10% FBS (Thermo Fisher Scientific, Cat. #10270-106).

    Techniques: Drug discovery, Over Expression, Derivative Assay, Control, Colony Assay, Cell Culture